Two bifunctional streptomyces - e . coli vectors were constructed that contained the phage lambda promoter ( pr ) upstream of the his6 - tagged recombinant pks gene 构建了两个链霉菌-大肠杆菌双功能pks表达质粒,在重组pks基因上游携带有噬菌体启动子。
2.
Integrated plasmids containing phage lambda promoter pr - directed and epitope - tagged 2 . 7 kb pks gene were constructed for tagging the natural fr - 008 pks with specific immunodeterminant ( epitope ) . these constructs were transferred into streptomyces sp 构建了带有噬菌体启动子和特异性抗原决定簇(表位)及2 . 7kbpks基因的整合型质粒,用于标记天然的fr - 008pks 。